01Product

L-Histidine HCl Monohydrate 83% L-Histidine

At a glance

L-Histidine HCl Monohydrate from Zhicheng Bio — supply summary

Zhicheng Bio supplies bulk L-Histidine HCl Monohydrate (83% L-Histidine), CAS 5934-29-2, shipping from Shanghai, China. This grade is produced to order. Every shipment carries a batch Certificate of Analysis; specification sheet and MSDS are supplied on request. Sourced to order — about 1 week to Shanghai port. Zhicheng Bio publishes no list price — quotes are issued per quantity and Incoterm by email to zhicheng1@zhicheng-bio.cn.

Supplier: Zhicheng Bio, Shanghai, China
CAS number: 5934-29-2
Molecular formula: C6H9N3O2·HCl·H2O
Grade / specification: 83% L-Histidine
Availability: Made to order
Documents: Batch CoA with every shipment; spec sheet and MSDS on request
Packing: 25 kg cartons or bags, adjustable
PubChem CID: 165377
How to get a price: Email RFQ to zhicheng1@zhicheng-bio.cn — no list price is published

Amino Acids ~1 week

L-Histidine HCl Monohydrate

83% L-Histidine

CAS 5934-29-2 · C6H9N3O2·HCl·H2O

Bulk L-Histidine HCl Monohydrate for B2B manufacturing — supplied against your target specification, with documentation your QA can review before anything ships. Pricing below is indicative; send an RFQ for a firm quote, MOQ and lead time.

$11.2 – $12.6 /kg by order volume · indicative, ex-Shanghai

Indicative range — larger volumes price toward the low end. Ingredient markets move weekly; your firm price is confirmed by quotation, typically within one business day.

Category: Amino Acids

Availability: Sourced to order — about 1 week to Shanghai port

Packing: 25 kg bags, cartons or drums (standard)

Key supply points

Supplied against your target specification — USP / FCC / JP monograph where applicable
Recent batch COA provided with the quotation, before you commit
Consolidates with other SKUs into one shipment out of Shanghai
Third-party lab verification available pre-shipment on request

Documentation with every batch

COA per batchSpec SheetMSDS / SDSExport docs

Third-party lab verification can be arranged pre-shipment on request. Certificates of origin, non-GMO or allergen statements per destination-market requirements.

Request COA & Quote Use the RFQ form

Shipping & Lead Time

Estimate freight for this product

~1 weekSourced to order — about 1 week to Shanghai port

Destination region Quantity (kg)

Air FreightFast

Transit: —
Est. freight *: —
Total door-to-port: —

Sea LCLValue

Transit: —
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Request Exact Quote

Documentation-first

COA per batch, Spec Sheet and MSDS — reviewed by your QA before anything ships.

Ready-stock depth

Multi-tonne inventory on core SKUs, plus 28 actives held in our US warehouse in Los Angeles for domestic delivery.

Certified supply chain

Sourced from audited, certified manufacturers — ISO, HACCP, Halal & Kosher available.

Flexible export

25 kg bags, cartons or drums; FOB / CIF / DDP by lane, sea or air consolidation.

02Identity & properties

Identity & properties

Compound identifiers and physical properties for this material. The binding version is always the Specification Sheet and batch COA issued with your quotation.

CAS No.	5934-29-2
Molecular Formula	C6H12ClN3O3 (written as C6H9N3O2·HCl·H2O)
Molecular Weight	209.63
EINECS	611-821-4
Synonyms	Histidine monohydrochloride monohydrate, L-Histidine HCl H2O, L-His-HCl monohydrate
Appearance	White crystalline powder
Solubility	Fairly soluble in water; insoluble in alcohol and ether (Merck Index, for the monohydrochloride). Free-base L-histidine for comparison: 45.6 g/L in water at 25 °C.
Pharmacopoeial reference	JP (as L-Histidine Hydrochloride Hydrate)

03Specifications

Specifications

Typical parameters below — the binding version is the Specification Sheet and batch COA we send with your quotation.

Parameter	Specification	Test method
Stated L-histidine content	83% (as printed on the offer sheet) — see verify note	Per supplier offer sheet
Water (as monohydrate)	—	Karl Fischer or loss on drying
Assay (as-is / dried basis)	—	Titration or HPLC per the applicable monograph
Appearance	—	Visual
Identification	—	IR / specific optical rotation per monograph
Specific optical rotation	—	Polarimetry
Loss on drying	—	Gravimetric
Residue on ignition / sulphated ash	—	Gravimetric
Chloride	—	Per monograph
Sulphate	—	Per monograph
Iron	—	Per monograph
Other amino acids / related substances	—	TLC or HPLC per monograph
Heavy metals (Pb / As / Cd / Hg)	—	ICP-MS or per monograph
Microbiology (TAMC / TYMC / E. coli / Salmonella)	—	USP <61>/<62> or equivalent

A dash means we have no fixed limit to publish for that parameter: it is reported on the batch COA and the limit is agreed with your QA. We would rather leave it blank than invent a number. Full physical, chemical, heavy-metal and microbiological parameters are listed in the Specification Sheet — sent with every quotation.

04Formulation & handling notes

Formulation & handling notes

The practical points our technical team raises with buyers of this material — the things that decide whether a batch behaves in your process.

Histidine is the only proteinogenic amino acid with a side-chain pKa in the physiological range — the imidazole group sits near pK 5.97 (Merck reports pK1' 1.78, pK2' 5.97, pK3' 8.97 for the free base). That is precisely why histidine is used as a buffer in biologics formulation, and it is also why a histidine addition will buffer your own product around pH 6 whether you wanted it to or not.

This is a monohydrate salt, so three different weights exist for the same material: the hydrated salt (209.63), the anhydrous salt (191.62) and the free base (155.15). Free histidine is 74.0% of the hydrated salt. Every potency calculation must state which basis it is on.

The hydrochloride form is chosen over the free base for solubility and handling — Merck describes the monohydrochloride as fairly soluble in water and insoluble in alcohol and ether, and the free base is only 45.6 g/L in water at 25 °C.

Water of crystallisation makes loss-on-drying a specification parameter rather than a purity check. A 'dry' lot that has lost its hydrate water will over-assay on the salt basis and under-deliver on a histidine-content basis.

In a beta-alanine stack, histidine is the co-substrate of the carnosine synthesis pathway, so the pairing is common commercially — but the two ingredients have very different solubility and taste profiles and should be evaluated separately in the flavour system.

05Applications

Applications

B2B manufacturing use only — no finished-product or health claims.

Free-form amino acid and EAA blends

Carnosine and beta-alanine stack formulations

Clinical, enteral and parenteral nutrition raw material

Buffer preparation and biopharmaceutical formulation (histidine is a common protein-formulation buffer)

Cell-culture media

06Regulatory status by market

Regulatory status by market

Status of the raw material, stated as conservatively as our sources allow. This is supply-chain information, not regulatory advice — confirm the position for your finished product and destination market with your own regulatory counsel.

Market	Status	Basis
United States	Listed by name in FDA's food-additive regulation for amino acids as 'L-Histidine'. That regulation permits use as a nutrient 'to significantly improve the biological quality of the total protein' of a food, subject to a cap of 2.4 percent by weight of the total protein of the finished food, and to the labelling requirements in paragraph (e). It is not a general-purpose clearance for any food use, and it is not the authority under which dietary supplements are marketed — those sit under DSHEA and 21 CFR part 111.	21 CFR 172.320(a), (b), (c)(4), (e)
European Union	Amino acids are not covered by the harmonised positive lists of Directive 2002/46/EC (Annexes I and II of that Directive cover vitamins and minerals only). Amino acid ingredients are therefore governed by general EU food law plus national rules, with mutual recognition under Article 4 of Regulation (EC) No 764/2008 in practice.	Directive 2002/46/EC (food supplements); national member-state rules
Japan	An Official Monograph titled 'L-Histidine Hydrochloride Hydrate' is listed in the Japanese Pharmacopoeia, Eighteenth Edition. Monograph existence only — no limits transcribed.	JP XVIII, Official Monographs
China	Status as a nutrient fortification substance / food additive under Chinese national standards to be confirmed.	GB 14880-2012 (nutrient fortification substances), GB 2760 (food additives), and NHC new-food-ingredient announcements

07Selected research

Selected research

Peer-reviewed literature on this compound, with linked identifiers so you can read the primary source rather than take our word for it.

These references are listed for scientific context only. They describe published research on the compound; they are not claims about this raw material and not claims about any finished product. Zhicheng Bio makes no medical, health or performance claims.

A review of histidine intake reported that supplementation at 4.0-4.5 g/day and increased dietary histidine intake have been associated with lower BMI, adiposity, markers of glucose homeostasis, pro-inflammatory cytokines and oxidative stress, and noted that studies report adverse effects at high intakes above roughly 24 g/day. Thalacker-Mercer AE, Gheller ME. J Nutr. 2020;150(Suppl 1):2588S-2592S. PMID 33000165 DOI 10.1093/jn/nxaa229 Source
A physiological review of carnosine identified L-histidine, and specifically its imidazole moiety, as the prime bioactive component of the dipeptide, with beta-alanine mainly regulating the rate of carnosine synthesis. Boldyrev AA, Aldini G, Derave W. Physiol Rev. 2013;93(4):1803-1845. PMID 24137022 DOI 10.1152/physrev.00039.2012 Source
A review of the side effects of amino acid supplements examined histidine among other amino acids and discussed competition for shared membrane carriers and degradative enzymes at high intakes. Holeček M. Physiol Res. 2022;71(1):29-45. PMID 35043647 DOI 10.33549/physiolres.934790 Source

08Packing, storage & lead time

Packing, storage & lead time

Standard packing	25 kg bags, cartons or drums
Private packing	Custom packing & labeling available for regular customers
Storage	Cool, dry place, sealed in original packaging
Lead time	Sourced to order — about 1 week to Shanghai port
Shipping	Sea or air consolidation out of Shanghai · FOB / CIF / DDP by lane

09Procurement FAQ

Procurement FAQ

01The listing says 83% L-histidine. Is that right?

That figure needs checking and we would rather flag it than let you build a label on it. For L-histidine hydrochloride monohydrate, free histidine cannot exceed 74.0% of the salt mass (155.15/209.63) — even the anhydrous salt is only 81.0%. The 83% almost certainly refers to an assay of the salt, not to histidine content. Ask us for the manufacturer's specification sheet and we will get the basis stated unambiguously before you order.

02Do I need the hydrochloride or the free base?

Take the hydrochloride when you need solubility and easy handling; take the free base when you want maximum histidine per kilogram and can accept lower water solubility (45.6 g/L at 25 °C). For buffer work the choice is usually driven by the pH you are targeting and whether you can tolerate chloride.

03Why does histidine change my product's pH more than other amino acids?

Because it is the one amino acid whose side chain buffers in the range you care about — the imidazole pK is around 6. That is the whole reason histidine is used as a formulation buffer for biologics. Expect it to pull your system toward pH 6 and plan your buffer accordingly.

04How should the water content be specified?

As a range, not as a maximum. It is a monohydrate: theoretical water is 8.6%. A lot that has dried out will read high on a salt-basis assay and low on histidine content. Ask for Karl Fischer water on the COA.

05What is the MOQ for L-Histidine HCl Monohydrate?

MOQ is quote-based and flexible. Tell us your target quantity in the RFQ — for core in-stock SKUs we can usually accommodate both trial orders and multi-tonne volumes.

06Can I get a sample of L-Histidine HCl Monohydrate before ordering?

Yes — samples are available on request for evaluation and lab verification. Mention it in your RFQ and we will confirm the arrangement.

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